I am making a project, and I want to be able to click through a sprite. This sprite takes up the whole space (it's partially transparent, so one can see what's underneath), and there are other sprites underneath it that I want to be able to click. If this isn't possible, please advise on what I should do instead to achieve what I'm after.

You could give the sprites a hitbox and see if the mouse pointer is down and colliding with the hitbox.

Use hitboxes. If the mouse is down (clicking) and touching the hitbox (basically touching the sprite) Probably not what you want, but there is no way for a sprite to be clicked or sensed through (mouse pointer) Many platformers use hitboxes for better movement and collision detection.

bruh ninja'd bc of new scratcher limit >:(